We further found that high expressions of GSDMD and GZMB displayed favorable prognosis in OC from six cohorts (P = 0.034 and P = 2.08E-5, respectively) (Fig. 9A), GSDMD and GZMB were significantly associated with the expression of immune checkpoint molecules with pan-cancer analysis (Additional file 8: Fig. S8B-C). The gene discussed is GSDMD; the disease is cancer.