CD274 and neoplasm: In HER2+ (Fig. 5d), the most negatively correlated purity statistics from different IHC markers were Lymph: CD163 purity and Lymph: CD8 purity (ρ = −0.79, P-value = 1.96e-14); Stroma: CD163 purity and All: CD8 purity (ρ = −0.73, P-value = 1.05e-11); Tumor: CD163 purity and Tumor: PD-L1 purity (ρ = −0.73, P-value = 2.46e-11).