To analyze the role of Notch pathways in HG-induced Nrg1 upregulation, Nrg1 levels were assessed following genetic and pharmacological inhibition of Notch pathways in breast cancer cells using siRNA-Rbpj and DAPT (N-[N-(3,5-Difluoroph enacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), respectively. This evidence concerns the gene RBPJ and breast carcinoma.