In nude mice, subcutaneous tumour tissues formed by TPX2 overexpressed HCC cells was able to reduce blood levels (plasma concentration) after sorafenib oral administration at a series of time points (2nd, 8th, 20th, 40th h), whereas in nude mice, intrahepatic tumour tissue formed by TPX2 overexpressed HCC cells was not able to affect blood levels after sorafenib oral administration at a series of time points (2nd, 8th, 20th, 40th h) (Supplemental Tables 1, 2). This evidence concerns the gene TPX2 and hepatocellular carcinoma.