To address the role of microglial Orai1 channels in regulating microglial Ca2+ signaling, neuroinflammation, and neuropathic pain following peripheral nerve injury, we generated a microglial-specific Orai1 KO mouse line by crossing Orai1fl/fl mice with the inducible Cre line, Cx3CR1-Cre/ERT2, for selective deletion of Orai1 in microglia in the CNS (31). Here, ORAI1 is linked to peripheral nerve injury.