To identify prognostic targets which affect the prognostic survival status of READ, survival analysis of key DNA methylation sites indicated that cg04441191 (MAP4), cg05658717 (KSR2), cg09622330 (GRIN2A), cg10698404 (YWHAG), cg17047993 (SPAG9), cg24504843 (CEP135), and cg24531267 (CEP250) were closely related to the prognostic survival (Figure 5). This evidence concerns the gene SPAG9 and reading.