Next, to investigate the effect of DNA methylation on downstream transcriptional expression, we applied Spearman correlation to calculate the association between DNA methylation β values and gene expression in the same cancer sample and found strong correlations between DNA methylation and transcription in MAP4, YWHAG, KSR2, SPAG9, and CEP135 (Table S6, Supplemental Digital Content, http://links.lww.com/MD/I368). This evidence concerns the gene KSR2 and cancer.