It was found that DNA methylation sites of 7 genes such as MAP4 (cg04441191), KSR2 (cg05658717), GRIN2A (cg09622330), YWHAG (cg10698404), SPAG9 (cg17047993), CEP135 (cg24504843), and CEP250 (cg24531267) were closely related to the prognosis of READ, which could be used as prognostic markers of READ. This evidence concerns the gene SPAG9 and reading.