In the current study, dietary supplementation with 10% fructose upregulated the expression levels of key genes involved in lipid biosynthesis (G6PC, glpK, dgkA, and ACSL) in peripheral adipose tissues and promoted more lipids synthesis, which suggested that overfed geese were protected against steatosis induced by fructose by depending on peripheral adipose tissues for de novo lipogenesis. The gene discussed is G6PC1; the disease is steatosis.