BALB/c mice intranasally challenged with AA extract and active secretory AChE derived from Nb had increased CXCL1/CXCL2 levels but reduced eosinophilia, M2 macrophages, and IL-5 and IL-13 levels in the lung tissue compared to both the PBS- and inactive AChE-treated controls. Here, ACHE is linked to Increased total eosinophil count.