Initially, a pseudouridylation-related gene list (PUS1, RPUSD3, TRUB1, PUS3, RPUSD4, RPUSD2, PUS10, PUS7, PUSL1, PUS7L, RPUSD1, DKC1, and TRUB2) was generated via literature mining, and then their expression profiling in the TCGA dataset for prostate adenocarcinoma (TCGA-PRAD) was investigated. This evidence concerns the gene RPUSD3 and prostate adenocarcinoma.