Initially, a pseudouridylation-related gene list (PUS1, RPUSD3, TRUB1, PUS3, RPUSD4, RPUSD2, PUS10, PUS7, PUSL1, PUS7L, RPUSD1, DKC1, and TRUB2) was generated via literature mining, and then their expression profiling in the TCGA dataset for prostate adenocarcinoma (TCGA-PRAD) was investigated. The gene discussed is RPUSD1; the disease is prostate adenocarcinoma.