In the present study, we illustrated a novel discrimination model of the low ratio of CD3+CD4+CD69+T/CD3+ CD8+CD69+T, PCT>0.53 ng/ml, and CO2CP<26.5 mmol/L, which could strongly predict the diagnosis of the G- sepsis (versus G+ sepsis), with an AUC of 0.947. The gene discussed is CD4; the disease is Sepsis.