As tumor necroptosis normally starts to happen in tumors at around 10 weeks in the MMTV-PyMT breast tumor model [30], we then collected tumors at 10, 12 and 15 weeks from WT and Mlkl KO MMTV-PyMT mice and examined the infiltrating T cell anti-tumor activity by measuring the levels of IFN-γ and the percentage of Granzyme B positive cells, two markers of CD8+ T cell functions and the levels of T-bet and IFN-γ (Th1) of CD4+ T cells [41]. This evidence concerns the gene IFNG and breast neoplasm.