PRF1 and melanoma: We found a higher expression of cytolytic markers in pTMB-high compared with TMB-high or aneuploidy-low tumors in both the TCGA (TMB, P > 0.05 for all genes; pTMB, P = 0.02 for GZMK, IFNG and PRF1; P = 0.04 for NKG7; aneuploidy, P > 0.05 for all genes; Supplementary Fig. 5) and ICB melanoma cohorts (GZMB: TMB P = 0.02, pTMB P = 6.1 × 10−3, aneuploidy P = 0.80; IFNG: TMB P = 0.01, pTMB P = 5.6 × 10−3, aneuploidy P = 0.88; PRF1: TMB P = 0.03, pTMB P = 5.1 × 10−3, aneuploidy P = 0.69; Fig. 5).