Here, we took advantage of iPSC-derived MNs and RNA profiling by digital color-coded molecular barcoding in soma and neurites to gain insight into the molecular mechanisms underlying axonal dysfunctions in ALS, with a specific focus on the interplay between three RBPs: ALS mutant FUS, FMRP and HuD. This evidence concerns the gene ELAVL4 and amyotrophic lateral sclerosis.