In this study, we used A549 lung cancer cells and noncancerous BEAS-2B epithelial cells as model systems and applied long-read ChIA-PET [24] to map global chromatin interactions associated with different factors, including RNA polymerase II (RNAPII), CCCTC-binding factor (CTCF), EZH2, and H3K27me3. Here, CTCF is linked to lung cancer.