With the exception of the ABCB1 gene (maker-scaffold10x_157_pilon-snap-gene-0.179), two EGF-like proteins (maker-scaffold10x_157_pilon-snap-gene-0.185 and maker-scaffold10x_157_pilon-snap-gene-0.196) and a serine rich protein (maker-scaffold10x_157_pilon-snap-gene-0.188), all the candidate genes were transcribed by the three major F. hepatica life cycle stages, namely newly excysted juveniles (NEJ), immature fluke 21 days post infection and adult fluke (with TPM values ranging from 2–510). This evidence concerns the gene ABCB1 and infection.