USP22 and myocardial infarction: Subsequent findings demonstrated that LVEF% and LVFS% were both reduced (Figure 2c, p < 0.05), myocardial fiber arrangement was disturbed, cardiomyocyte interstitial edema was augmented and inflammatory cell infiltration was enhanced (Figure 2d, p < 0.05), while myocardial infarction area was increased (Figure 2e, p < 0.05) and levels of CK-MB, cTnI and LDH levels in the serum were elevated following USP22 silencing (Figure 2f, p < 0.05), indicating that USP22 ablation could reverse the protective effects of SPC on MI/RI rats.