As Pd-1 was upregulated the highest on murine TILs in comparison to Ctla-4 or Lag-3 (Figure 3B), we chose to block the Pd-1/Pd-l1 axis in order to enhance trAb-mediated anti-tumor immune response, as NXS2 cells are known to constitutively express Pd-l1 (25). This evidence concerns the gene PDCD1 and neoplasm.