The qPCR results revealed that the expression of CNDP2 was high in four PC cell lines (LNCap, PC-3, C4-2, and 22RV-1), and the expression of SERPINH1 was high in immortalized prostate cell lines like RWPE-1 compared with four prostate cancer cell lines (Figures 3C, D). This evidence concerns the gene CNDP2 and pachyonychia congenita.