To further determine the critical roles of NFAT2 in KD, 40 ng/ml TNFα was used to stimulate HCAECs to mimic vasculitis in vitro. The dose of TNFα was determined by the expression of NFAT2 after HCAECs were stimulated by different doses (Supplementary Figure 1A). The gene discussed is NFATC1; the disease is vasculitis.