BBOX1 and hepatocellular carcinoma: Consistent with the confocal microscopy results, transmission electron microscopy (TEM) images and western blot assays revealed that the combination of BBOX1-AS1 overexpression and sorafenib promoted autophagy in HCC cells, whereas BBOX1-AS1 silencing weakened the effect of sorafenib on autophagy (Figures 3D and 3E).