ESR1 and neoplasm: As a result, compared with TAM, 17 novel significant functions enriched in DEMs by wTAM were depicted in Figure 1B, of which Cell adhesion (FDR = 2.28E−3 in wTAM; FDR = 0.062 in TAM) and Cytoskeleton remodeling (FDR = 6.10E−3 in wTAM; FDR = 0.21 in TAM) are the crucial processes for breast cancer metastasis (Fridrichova and Zmetakova, 2019; Scully et al., 2012), which suggested the distinct differences in tumor progression and invasion between ER− and ER+ subtype.