BAIAP2 and glioblastoma: In order to quantitate the role of IRSp53 at the interface of membrane dynamics and cytoskeletal contractility, we generated the IRSp53 knockout (KO) U251 glioblastoma cells (Figure S1, Supporting Information) using a CRISPR/Cas9 system, as described previously.[13] We first inquired if IRSp53 depletion caused any effects easily observable under a microscope.