Western blot experiments showed that the expression level of IGF-1R was lower in normal hepatocyte lines THLE-2 and HHL-5 than in HCC cell lines SK-Hep1, HepG2, and Huh-7, where it was highest in SK-Hep1 (Fig. 1 A and 1B). Here, IGF1R is linked to hepatocellular carcinoma.