For ICC assays, cells were stained for surface markers CD3 (BV711-labeled antibodies), CD4 (APC-labeled antibodies), and CD8 (APC Cy7-labeled antibodies) and for the presence of intracellular cytokines with antibodies against IFN-γ (FITC-labeled antibodies), TNF-α (PE Dazzle 594-labeled antibodies), IL-2 (PE-labeled antibodies), IL-4 (BV605-labled antibodies), and IL-5 (BV421-labeled antibodies). Here, CD4 is linked to intrahepatic cholangiocarcinoma.