To study further how HS and lamin phosphorylation affect nuclear and lamina structure, HeLa cells, primary human dermal fibroblasts from a healthy individual and from a dilated cardiomyopathy patient carrying the p.S143P mutation in LMNA, as well as primary mouse fibroblasts were imaged and analyzed in detail upon HS. The gene discussed is LMNA; the disease is dilated cardiomyopathy.