To validate whether expression of the three candidate genes (TNFSF4, TNFRSF18, and IL12RB2) in the tumor microenvironment is implicated in NK cell migration toward cancer cells, we conducted a Transwell migration assay using GBM U87MG cells, T cells, and NK cells derived from PBMCs. The gene discussed is TNFRSF18; the disease is cancer.