To establish whether social and cognitive deficits in ChatCRECul3F/+ mice may be caused, at least in part, by the decreased cortical neuron activity as a result of the reduced BF cholinergic projection, we delivered a retrograde rg-AAV-hSyn-DIO-hM3D(Gq)-mCherry (rg-Gq) DREADD virus into the PFC. This evidence concerns the gene C4A and Cognitive impairment.