Similarly, the frequency of CD8+GZMB+ CTLs is the highest (27.6 ± 3.7% out of all CD8+ T cells) for the ICIE group (Fig. 6d & Supplementary Fig. 16e, f), which is confirmed by immunofluorescence staining images (Fig. 6e and Supplementary Fig. 16g) showing the distribution of CD8+GZMB+ cells in tumor sections. This evidence concerns the gene CD8A and neoplasm.