As a quality control of the ChIP samples, ChIP-qPCR confirmed the interaction of GRHL2 with the promoter region of CLDN4, a known direct target gene of GRHL2 [4] in all three luminal, GRHL2-positive cell lines but not in the GRHL2-negative Hs578T human basal-B breast cancer cell line (Additional file 2: Fig. S2). The gene discussed is CLDN4; the disease is breast carcinoma.