We then used qRT-PCR and ELISA to verify the prominent hypersecretion of cytokine CCL3 in supernatants collected from the THLE3 cell line after co-culture with the ICC cells (Fig. 2b and d), while a slightly elevated level of CCL23 secretion was observed in Fig. 2c. The gene discussed is CCL23; the disease is intrahepatic cholangiocarcinoma.