CTH and Hirschsprung disease: Previous studies have focused on the role of Cth in the repair of intestinal barrier damage due to intestinal inflammation, and a small number of clinical studies showed that reduced expression of cystathionine-β-synthase (CBS) and Cth in patients with Hirschsprung disease may affect mucosal integrity and colonic contractility, which are possible causes of hirschsprung-associated enteritis [14].