To verify the hypothesis that the hyperexcitability of PNs in PL guides autism-like behaviors, we expressed AAV-CaMKIIα-hM4D(Gi)-mCherry in the PL of 4-week-old WT and Tmem74−/− mice (Fig. 2a, b) under intraperitoneal (i.p.)injection of clozapine-N-oxide (CNO, 1 mg/kg) [40]. The gene discussed is TMEM74; the disease is autism.