TMEM74 and Anxiety: To further confirm the hypothesis that a Tmem74 deficit in the PL mediated social deficits and anxiety-like behaviors, pAAV2/9-CaMKIIα-EGFP-Tmem74-3Flag or pAAV2/9-CaMKIIα-EGFP-3Flag was injected respectively into the PLs of 4-week-old WT and Tmem74−/− mice (Fig. 6a).