Cancer cell subcluster 1 was enriched in TNFα signalling via NF-kB; cancer cell subclusters 2 and 6 were enriched in the E2F and MYC signalling pathways; cancer cell subcluster 3 was enriched in the hedgehog, MYC and oxidative phosphorylation signalling pathways; cancer cell subcluster 4 was enriched in the TNFα via NF-kB, IFN-α and KRAS signalling pathways; cancer cell subcluster 5 was enriched in the epithelial-mesenchymal transition and angiogenesis signalling pathways; and cancer cell subcluster 7 was enriched in the MYC and oxidative phosphorylation signalling pathways. This evidence concerns the gene NFKB1 and cancer.