To that end, three experiments were performed, namely (i) the quantification of FosB protein expression within the ACC and amygdala, (ii) the effects of chemical inhibition (through microinjection of CoCl2, a non-specific synaptic inhibitor) of the ACC and amygdala on the anxiety response of mice housed in pairs with a conspecific subjected to chronic restraint stress. The gene discussed is FOSB; the disease is Anxiety.