In this study, the cytological morphology of CD66b+CD10–-NDN in sepsis patients was band cells (88 ± 7%) and metamyelocytes (12 ± 4%), while cytological morphology of CD66b+CD10–-LDN was band cells (12 ± 6%), metamyelocytes (64 ± 12%) and myelocytes (24 ± 5%), which means although the cytological phenotype of neutrophils in CD66b+CD10–-NDN and CD66b+CD10–-LDN were all immature, the left shift of nucleus was increased and led to the separating in the LDN after blood density gradient centrifugation. The gene discussed is MME; the disease is Sepsis.