Some researchers reported that in the case of cancer (20, 26), HIV-1 infection (27), pregnancy (28), and granulocyte colony-stimulating factor (G-CSF) (8), it might be difficult to distinguish mature neutrophils in immature ones by CD16 and CD11b and no scholar has studied the homogeneity and heterogeneity of NDN and LDN subgroups in sepsis patients (17, 22, 29–31). This evidence concerns the gene ITGAM and HIV-1 infection.