A total of 24 patients were excluded after the primary assessment due to the following reasons: positive history of cardiovascular diseases (CVDs) (n = 9), owning cardiac risk factors including obesity, smoking, DM, HTN, HLP, and positive family history (n = 12), and prior surgical or pharmaceutical (anti-TNF or anti-IL) intervention (n = 3). The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.