Figure 8A–C shows the top eight cancers with the strongest correlation between DEF6 expression and ESTIMATE score, immune score, and stromal score. We discovered that the three immune infiltration scores significantly increased in GBM, LGG, SARC, KIRP, KIRC, LIHC, and KICH with an increase in level of expression of DEF6, suggesting that DEF6 expression may be crucial for immune infiltration in these malignancies. Finally, Figure 8D provides an overview of the correlation between DEF6 expression and immune infiltration in 32 cancers. The gene discussed is DEF6; the disease is cancer.