In clear cell renal cell carcinoma, a high level of expression of DEF6 predicts poor prognosis (7); in human osteosarcoma, a high level of expression of DEF6 is associated with metastasis and poor prognosis (8); in ovarian carcinoma, a high level of expression of DEF6 is associated with poor prognosis (9). Here, DEF6 is linked to osteosarcoma.