High serum levels of free IGF-I levels have indeed been shown to predict AGT, which is in line with our findings of high total IGF-I (positively correlated with free IGF-I) and low IGFBP-1 (negatively correlated with free IGF-I) as predictors of prediabetes and T2D in women (18, 53). This evidence concerns the gene IGFBP1 and prediabetes syndrome.