In this study investigating the risk for healthy middle-aged NGT men and women, many with a positive FHD, to develop prediabetes or overt T2D over a period of 8-10 years, we focused on biomarkers of insulin sensitivity and insulin production, i.e. adiponectin, IGFBP-1, IGFBP-2, IGF-I and IGF-II; proteins being linked to AGT (5–10, 15–18, 21, 22, 25–29). This evidence concerns the gene INS and prediabetes syndrome.