CYP20A1 and metabolic dysfunction-associated steatotic liver disease: To validate the common DEGs at the transcriptome level in NAFLD, we compared qRT-PCR data with RNA-seq data from the same samples, including CYP20A1, CYP2U1, SLC9A6, SLC26A6, SLC31A1, SLC46A1, SLC46A3, SULT1B1, and UGT2A3. We noted that RNA-seq data from all nine DEGs showed a positive correlation with the data obtained by the qRT-PCR technique (Figure 7).