It was found that only BAX, CHMP4A, CHMP4B, CYCS, ELANE, GSDME, HMGB1, IL18, CASP9, PJVK, and TNF had no mutations, and others showed mutations in HNSCC tissues (Figure 2A). The gene discussed is HMGB1; the disease is head and neck squamous cell carcinoma.