Nine genes associated with HNSCC prognosis (TTLL1, TRIML2, DYNC1I1, KLHL35, CAMK2N1, TNFRSF18, GLDC, SPINK5, and DKK1) were used to construct a new prognostic signature for HNSCC by LASSO regression algorithm and univariate Cox regression. Here, KLHL35 is linked to head and neck squamous cell carcinoma.