Based on the screening conditions of p < 0.05 and Spearman correlation coefficient >0.3, two DNA methylation sites (cg03559467: correlation coefficient = −0.303, p < 0.001 and cg25841477: correlation coefficient = −0.334, p < 0.001) might be related to the transcription of ZNF281 in PAAD (Figures 5E, G). The gene discussed is ZNF281; the disease is pancreatic adenocarcinoma.