In the subgroup analysis, MTHFR rs1801133 (677C>T) gene polymorphism was associated with stroke susceptibility in Asian (OR = 1.46; 95%CI = 1.30–1.63; p < 0.001), Hispanic or Latin populations (OR = 2.09; 95%CI = 1.50–2.95; p < 0.001), middle-aged (OR = 1.50; 95%CI = 1.27–1.78; p < 0.001), and elderly groups (OR = 1.23; 95%CI = 1.08–1.40; p = 0.001). The gene discussed is MTHFR; the disease is Stroke.