We further performed GSEA analysis and found that RNF169 was involved in the regulation of inflammation and immune pathways, including RIG-1-like receptor pathway, Toll-like receptor signaling pathway, cytosolic DNA sensing pathway, steroid hormone biosynthesis, regulation of autophagy and autoimmune thyroid disease pathways (Supplementary Figure S7). The gene discussed is RNF169; the disease is autoimmune thyroid disease.