For the top 10 mutated genes in PAAD, including KRAS, TP53, TTN, SMAD4, MUC16, CDKN2A, RYR1, RNF43, ARID1A and GNAS, missense mutations occurred with the highest frequency (Supplementary Figure S5F). The gene discussed is RNF43; the disease is pancreatic adenocarcinoma.