KR158B, KR158B CCL2 KD or KR158B CCL7 KD glioma cell lines were implanted in Ccr2+/RFP/Cx3cr1+/GFP mice, and flow cytometry analysis of tumors and bone marrow was conducted 4.5 weeks post-implantation (Supplementary Figure 7). This evidence concerns the gene CX3CR1 and central nervous system cancer.