TSPAN4 and cancer: To explore the diagnostic value of TSPAN4 expression in pan-cancer, ROC analysis was used, suggesting that the TSPAN4 expression can be credible biomarkers in LUSC, UCS, THYM, KIRC, PAAD, OV, DLBC, CESC and UCEC (AUC > .9), and TSPAN4 expression can also be potential biomarkers in LUAD, LIHC, PRAD, KICH, ESCA, BLCA, GBM, KIRP, BRCA, READ and COADREAD (AUC > .8) (Figure 4).