miR-135b, miR-155, and KRAS were increased in the colon cancer mice group compared to the control group in both the plasma and the colon tissue samples. The probiotics consumption decreased their expression. Moreover, the miR-26b, miR-18a, APC, PU.1, and PTEN expressions were decreased in the colon cancer mice group compared to the control group and the consumption of the probiotics increased their expressions. Here, SPI1 is linked to malignant colon neoplasm.