To determine the role of Pin1 in the regulation of EMT in cervical cancer by matrix stiffness, we upregulated Pin1 expression in hela cells using an expression plasmid and inhibited Pin1 expression in hela cells using the Pin1 inhibitor Juglone38, 39 and verified its effect using western blotting (Figure 3c,d). The gene discussed is PIN1; the disease is cervical carcinoma.