Overall, our results demonstrated that the infection caused by single or mixed T. cruzi infection with different parasite genotypes induce a differential immune CD8+ T cell response quality, which suggest that the quality of CD8+ T cell response might dictate differences in the parasite control and infection outcomes at the chronic T. cruzi stage (Mateus et al., 2017; Mateus et al., 2021). The gene discussed is CD8A; the disease is infection.