CD4 and benign neoplasm: In a study involving 11 different donors, roughly 12% of the CD4+ cells were CD3+ CD4+ CD8− CD25+ CD127− with 85% of these cells expressing the FoxP3.46 This approach is an exciting alternative to isolate large numbers of Tregs; however, it is so far limited to pediatric heart transplant recipients or when third-party Tregs are utilized.