Subsequently, all gene-modifying regulators with the highest prognostic ability were screened by LASSO Cox regression analysis (Fig. 6B, C), which identified the following four genes to estimate the risk of colon adenocarcinoma: heat shock transcription factor 4 (HSF4); uroplakin 3B (UPK3B); zinc finger family member 767, pseudogene (ZNF767P); and ArfGAP with GTPase domain, ankyrin repeat and PH domain 9 (AGAP9) (Fig. 6D). This evidence concerns the gene UPK3B and colon adenocarcinoma.